Normal human colon cells suppress malignancy when fused with colon cancer cells |
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Authors: | Teresa L. Johnson Mary Pat Moyer |
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Affiliation: | (1) Depts. of Cellular and Structural Biology and Surgery, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284 San Antonio, Texas |
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Abstract: | Summary Normal human colon mucosa cells and cells obtained from histologically normal tissues near that cancer were fused with human colon cancer cells. Resultant hybrid populations of normal and malignant cell fusions behaved as nonmalignant cells in culture, were unable to grow in soft agar, did not express tumor-associated antigens, and were nontumorigenic in nude mice. Autofusion of the cancer cell population led to a phenotype intermediate between normal and malignant cells. That is, the cultures had a much lower plating efficiency in soft agar, and the tumors had a longer latency and slower growth rate in nude mice. This is the first cell culture system to demonstrate that normal epithelial cells can suppress malignancy of their autologous cancer cells, and is a prelude to more extensive studies of genetic events involved in malignant conversion of human colonic epithelium. This study was supported by The University of Texas Health Science Center at San Antonio Center for Human Cell Biotechnology and a graduate student stipend (T. J.) from the Department of Cellular and Structural Biology. |
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Keywords: | colon cancer tumor suppression anti-oncogenes cell fusion epithelial cells |
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