α-glucosidase in Mycoplasma mycoides subspecies capri |
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Authors: | BJ Wadher RJ Miles |
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Institution: | Department of Microbiology, King's College, University of London, Campden Hill Road, London W8 7AH, UK |
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Abstract: | Abstract Mycoplasma mycoides subsp. capri utilisede maltose in medium lacking serum and hence serum saccharolytic enzymes. The presence of α-glucosidase activity was demonstrated by p-nitrophenyl-α- d -glucoside hydrolysis in toluene-treated cells. Specific activities were approx. 4-fold higher in cells grown in the presence of maltose than in cells grown with other sugars or with glucose plus maltose. Extracellular activity was < 2% of cellular activity in growing cultures. α-Glucosidase activity was also demonstrated in cells grown in medium containing serum. It is suggested that the presence of α-glucosidase might be of value in mycoplasma chatacterisation; in a previous study, α-glucosidase activity was not detected in Mycoplasma mycoides subsp. mycoides . |
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Keywords: | Mycoplasma mycoides Characterisation Enzyme induction Catabolite repression |
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