Pseudomonas aeruginosa PAO1 as a model for rhamnolipid production in bioreactor systems |
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Authors: | Markus Michael Müller Barbara Hörmann Christoph Syldatk Rudolf Hausmann |
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Institution: | 1. Institut für Bio-und Lebensmitteltechnik, Technische Biologie, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany
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Abstract: | Rhamnolipids are biosurfactants with interesting physico-chemical properties. However, the main obstacles towards an economic
production are low productivity, high raw-material costs, relatively expensive downstream processing, and a lack of understanding
the rhamnolipid production regulation in bioreactor systems. This study shows that the sequenced Pseudomonas aeruginosa strain PAO1 is able to produce high quantities of rhamnolipid during 30 L batch bioreactor cultivations with sunflower oil
as sole carbon source and nitrogen limiting conditions. Thus PAO1 could be an appropriate model for rhamnolipid production
in pilot plant bioreactor systems. In contrast to well-established production strains, PAO1 allows knowledge-based systems
biotechnological process development combined with the frequently used heuristic bioengineering approach. The maximum rhamnolipid
concentration obtained was 39 g/L after 90 h of cultivation. The volumetric productivity of 0.43 g/Lh was comparable with
previous described production strains. The specific rhamnolipid productivity showed a maximum between 40 and 70 h of process
time of 0.088 gRL/gBDMh. At the same time interval, a shift of the molar di- to mono-rhamnolipid ratio from 1:1 to about 2:1 was observed. PAO1
not only seems to be an appropriate model, but surprisingly has the potential as a strain of choice for actual biotechnological
rhamnolipid production. |
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