RGD peptide-induced apoptosis in human leukemia HL-60 cells requires caspase-3 activation |
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Authors: | CD Anuradha S Kanno S Hirano |
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Institution: | (1) Regional Environment Division, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, Japan |
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Abstract: | RGD motif-containing peptides have been used in various studies of cell adhesion and growth. We report that RGD triggered
apoptosis at a concentration of 1 mmol/L, whereas RAD-containing peptides failed to induce apoptosis in HL-60 cells. RGD-treated
cells revealed internucleosomal DNA fragmentation. Western blot reveals caspase-3 activation in RGD peptide-treated cells.
A caspase-3 inhibitor z-VAD-FMK completely blocked the apoptosis, but a caspase-1 inhibitor (Ac-YVAD-CMK) and caspase-2 inhibitor
(z-VDVAD-FMK) did not block the apoptosis, suggesting that caspase-3 might have a critical role in the execution process of
apoptosis induced by RGD. RGD peptides have been used extensively to inhibit tumor metastasis. Our results should help in
further understanding the RGD peptide-induced apoptosis, which is important since RGD peptides have a potential role in therapies
of the future.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | apoptosis RGD HL-60 caspase-3 |
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