脑缺血／再灌流脑微血管内皮细胞ELAM—1和ICAM—1mRNA表达的研究

目的：探讨ELAM-1和ICAM-1在局部脑缺血/再灌流炎性反应过程中的作用。方法：采用厅局级龙线栓堵大脑中动脉造成局部脑缺血/再灌流模型,用RT-PCR方法检测缺血侧脑组织缺血/再灌流不同时间点ELAM-1和ICAM-1mRNA的表达。结果：假手术组脑组织未见ELAM-1和ICAM-1mRNA的表达,手术组非缺血侧脑组织仅见少量表达。脑缺血/再灌流后1h,缺血侧脑组织ELAM-1和ICAM-1mRNA的表达量已开始升高;再灌流后3h,ICAM-1mRNA的上调达高峰,而ELAM-1mRNA的上调在缺血/再灌流后6h达高峰,且持续至缺血/再灌流后48h。结论：EL-AM-1和ICAM-1参与了局部缺血再灌流脑组织损伤的病理过程。二者在白细胞进入缺血区脑组织的病理过程中发挥着重要作用。

Study on exprssion of ELAM-1 and ICAM-1 mRNA on microvascular endothelial cells during focal cerebral ischemia/reperfusion

Aim:To evaluate the role of ELAM 1 and ICAM 1 in the course of inflammatory reactions during focal brain ischemia/reperfusion.Methods: The focal brain ischemia/reporfusion model is carried by occluding middle cerebral artery.The expression of ELAM 1 and ICAM 1 mRNA after ischemia/reperfusion was evaluated with RT PCR.Results: No ELAM 1 and ICAM 1 mRNA were detected in the sham operated cortex and only little in the nonischemic cortex.The expression of ELAM 1 and ICAM 1 mRNA were upregulated at 1 hour,peaked at 6 hour and 3 hour respectively and remained elevated for up to 48 hours after ischemia/reperfusion.Conclusion: ELAM 1 and ICAM 1 participate in brain injury during focal ischemia/reperfusion and both of them play an important role in leukocyte infiltration into the ischemic tissues.