Solution structure of a SRP19 binding domain in human SRP RNA |
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Authors: | Sakamoto Taiichi Morita Satoshi Tabata Kazutoshi Nakamura Kouji Kawai Gota |
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Institution: | Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan. |
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Abstract: | Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop. |
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