Expression of a glucocorticoid receptor (DlGR1) in several tissues of the teleost fish Dicentrarchus labrax |
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Authors: | Vazzana M Vizzini A Salerno G Di Bella M L Celi M Parrinello N |
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Affiliation: | Laboratory of Marine Immunobiology, Department of Animal Biology, University of Palermo, Via Archirafi 18, Palermo, Italy. |
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Abstract: | Since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mRNA expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (DlGR1) in several Dicentrarchus labrax organs are reported. Riboprobe and specific antibodies were prepared by using the DlGR1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. Both mRNA and receptor were identified in head kidney, spleen, gills, intestine, heart and liver tissues. The functional roles of DlGR1 localization are discussed. |
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Keywords: | Glucocorticoid receptors In situ hybridization Immunohistochemistry Stress Dicentrarchus labrax |
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