The dose-response to testosterone propionate of preputial glands, pheromones and aggression in mice

Urine from female mice injected with 24 daily doses of 0, 1, 10, 50, and 100 μg testosterone propionate (TP) was applied to the coat of castrate males, and the aggressive response of fighter mice toward these castrates recorded. Some antiaggressive property in castrate female urine was confirmed, and this was neutralized by only 1 μg daily TP. Urine from the 50 μg group greatly increased the aggression response, but 100 μg had no additional effect.