Arginine stimulated insulin and glucagon release from islets transplanted into the liver of diabetic rats

We investigated the ability of intraportal transplanted islets to release insulin and glucagon after stimulation with arginine. Furthermore, the islet volume and hormone content of the recipient pancreas were analyzed. Three months after syngeneic portal islet transplantation the liver of STZ-diabetic rats was perfused in vitro in the presence of different arginine concentrations. Transplanted islets preserve their functional integrity for at least three months indicated by a stimulus adequate insulin release and contribute substantially to the observed amelioration of the diabetic state. The islet and B-cell volume as well as the insulin and glucagon content of the recipient pancreas are still markedly decreased three months after islet transplantation when compared with healthy controls.