5-Lipoxygenase from rat PMN lysate |
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Authors: | Mark T. Skoog James S. Nichols Jeffrey S. Wiseman |
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Affiliation: | Merrell Dow Research Institute, Cincinnati, Ohio 45215, USA |
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Abstract: | The products of arachidonic acid metabolism in the 15,000xg supernatant of sonicated rat PMN are described. Only products derived from 5-lipoxygenase are observed. These products are 5-HETE and products derived from hydrolysis of LTA4, particularly LTB4. Some minor products derived from decomposition of 5-HPETE are also observed. The dependence of the activity of 5-lipoxygenase on enzyme and on substrate concentrations is presented and discussed in terms of a kinetic model that includes enzyme inactivation during turnover and substrate inhibition. The 5-lipoxygenase activity is stimulated by Ca++ and ATP. |
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