表皮生长因子对neu基因表达的诱导作用(简报)

Shih等首次通过NIH/3T3细胞转染试验在乙基亚硝脲(ENU)诱导的大鼠神经胶质纤维瘤中分离鉴定出一种转化基因,称之为neu基因,其表达可导致培养的NIH/3T3细

THE EFFCT OF EPIDERMAL GROWTH FACTOR ON THE EXPRESSION OF erb B 2/NEU ONCOGENE IN MOUSE EMBRYO FIBROBLAST CELLS

The erb B 2/neu oncogene encodes a protein which sequence is closely similar to the epidermal growth factor receptor (EGFR). We have previously found that EGF can induce the expression of erb B 1/ EOFR gene in normal and ~3H-TdR transformed C3H/10T 1/2 CL 8 mouse embryo fibroblast cells i. e. NC3H10 and TC 3 H 10 respectively, but we do not know whether the neu oncogene expression can be induced by EGF. In this study, the effect of EGF on NC 3 H 10 and TC 3 H 10 has been observed by Northern blot analysis. The result indicated that EGF had a obvious induction effect on neu oncogene expression in these cells. Thus, the expression of both erbB1/EGFR gene and erbB 2/neu oncogene can be induced by EGF. This result may provide a novel clue to the molecular mechanism of EGF action in cell nucleus.