| 疏绵状嗜热丝孢菌原生质体的制备与再生 |
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| 引用本文： | 周庆新,陈静,于恺,李多川. 疏绵状嗜热丝孢菌原生质体的制备与再生[J]. 菌物研究, 2006, 4(2): 1-5 |
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| 作者姓名： | 周庆新 陈静 于恺 李多川 |
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| 作者单位： | 山东农业大学环境生物系,山东,泰安,271018;山东农业大学环境生物系,山东,泰安,271018;山东农业大学环境生物系,山东,泰安,271018;山东农业大学环境生物系,山东,泰安,271018 |
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| 基金项目： | 国家自然科学基金资助项目(30571246,30270013) |
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| 摘 要： | 以疏绵状嗜热丝孢菌(Thermomyces lanuginosus)为供试菌株,研究了菌龄、酶的种类及浓度、酶解时间、酶解温度和稳渗剂对原生质体制备的影响及稳渗剂对原生质体再生的影响。结果表明,制备嗜热丝孢菌原生质体比较适宜的条件为:PDB液体培养基培养28 h,以0.7 mol/L NaCl为稳渗剂,0.15 mol/L的溶壁酶,30℃酶解4 h。原生质体再生以0.7 mol/L蔗糖作稳渗剂为最佳。
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| 关 键 词： | 嗜热真菌 疏绵状嗜热丝孢菌 原生质体 制备 再生 |
| 文章编号： | 1672-3538（2006）02-0001-05 |
| 收稿时间： | 2006-03-30 |
| 修稿时间： | 2006-03-30 |
Protoplast Preparation and Regeneration of Thermomyces Lanuginosus |
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| ZHOU Qing-Xin,CHEN Jing,YU Kai,LI Duo-chuan. Protoplast Preparation and Regeneration of Thermomyces Lanuginosus[J]. Journal of Fungal Research, 2006, 4(2): 1-5 |
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| Authors: | ZHOU Qing-Xin CHEN Jing YU Kai LI Duo-chuan |
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| Abstract: | The effects of some factors on the preparation and regeneration of protoplasts of Thermomyces lanuginosus were studied,including the effect of the hyphal age,enzymes and their concentration,time and temperature of digestion,osmotic stabilizers on the protoplast preparation and the effect of osmotic stabilizers on the protoplast regeneration.The results indicated that the optimum conditions for preparing protoplasts were: the condia cultured in liquid PDB medium for 28 hours,0.7 mol/L NaCl used as the osmotic stabilizer,0.15 mol/L Lywallzyme used to digest the hyphae for 4 hours at 30℃.And(0.7 mol/L) sucrose was the osmotic stabilizer for the regeneration of protoplasts. |
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| Keywords: | thermophilic fungis Thermomyces lanuginosus protoplast preparation regeneration |
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