大鼠动脉粥样硬化过程中抗HSP70抗体水平变化及其意义

目的：观察高脂诱导大鼠动脉粥样硬化形成过程中血浆HSP70抗体水平及其与动脉粥样硬化的关系。方法：将28只大鼠分为正常组和高脂组,动态测定了大鼠血清TC、TG、LDL,HE染色观察大鼠主动脉弓病理改变;通过ELISA方法检测大鼠血浆HSP70抗体水平及抗体亚型的变化。结果：2周时,高脂组大鼠血清TC、LDL显著升高,与对照组比较具有显著性差异（P〈0．01）;而TG水平显著降低（P〈0．01）。血浆HSP70抗体在第四周开始显著升高（与对照比较P〈0．01）,随着动脉粥样硬化的进程逐渐升高;HSP70抗体IgM亚型第四周达到峰值（与对照比较P〈0．01）;而IgG亚型第四周开始升高（与对照比较P〈0．01）,后逐渐升高。第12周时主动脉出现粥样斑块典型的动脉粥样硬化的病理改变．结论：高脂可以诱导大鼠动脉粥样硬化形成,高脂组大鼠血浆HSP70抗体IgG亚型水平随着疾病的进程逐渐升高,与动脉粥样硬化具有显著的相关性,表明血浆HSP70抗体与动脉粥样硬化发生发展具有密切关系。

Level and effect of anti-HSP70 antibody changed in the procession of rat atherosclerosis

Aim： To explore the level of anti-HSP70 antibody in plasma during atherosclerosis procedure induced by high-fat diet in rat and the rela- tionship of them. Methods： Twenty eight rat were divided into high-fat diet group （H） and control group （C）. The total cholesterol （TC）, Glyceride（TG）, low density lipoprotein cholesterol（LDL-C） in serum, pathology change of rat Arch of the aorta were determined, the level of anti-HSP70 antibody and their Phenotype were evaluated by ELISA. Results： After two weeks, the serum concentrations of TC and LDL-C in rat supplemented by high-fat diet were significantly higher than those in control group（ P 〈 0.01）, the serum TG were much lower than those in control group（P 〈 0.01）. Four weeks later the level of anti-HSP70 antibody, IgM, IgG phenotype were significantly higher than those in control group（ P 〈 0.01 ）. There were lipin deposition and mottling formation in rat Arch of the aorta in rat supplemented by high-fat diet in 12th week. Conclusion： Atherosclerosis could be induced by high-fat diet in rat. Accompany with the atherosclerosis procession, the level of anti-HSP70 antibody was continuously elevated, the level of anti-HSP70 antibody was related to atherosclerosis. The level of anti-HSP70 antibody was closely associated with atherosclerosis.