兔肠缺血／再灌注损伤的超微结构改变及红花的保护作用

目的：观察红花注射液对兔肠缺血／再灌注损伤超微结构的影响,探讨其机制。方法：复制在体兔肠缺血／再灌注损伤模型。30只日本大耳兔,随机均分为3组（n=10）：假手术组（S组）,缺A／再灌注组（I／R组）和缺血／再灌注＋红花注射液组（SI组）。使用电镜观察各组肠组织标本超微结构的改变,作对比分析。结果：L／R组多数肠粘膜上皮细胞肿胀,胞质内液泡增多,大多数线粒体呈不同程度的肿胀,严重者可见嵴减少或消失,内质网扩张较明显,细胞表面微绒毛数量明显减少且排列较乱,部分微绒毛有肿胀、融合现象,上皮细胞间隙扩大,连接较疏松。粘膜下间质可见少量的浆细胞浸润现象,部分毛细血管周围可见水肿现象。SI组在上述部位的病理改变均明显减轻。结论：红花能有效减少炎性渗出,抑制微循环通透性的增加,阻断肠缺血／再灌注损伤进展的病理生理过程,对肠组织超微结构有良好保护作用。

Effect of Safflor injection on the intestine ultrastructure characteristics following intestine ischemia/reperfusion injury in rabbits

Aim： To explore effects of Safllor（ Chinese Tradional Medicine） on the intestine uhrastructure characteristics during intestine ischemia/ reperfusion injury（I/RI） in rabbits. Methods： Thirty rabbits were randomly divided into three groups ： control group（group S）, ischemia/ repeffusion group （group I/R） and Sattlor injection group （group S1） . Morphological changes of intestine ischernia/reperfusion in rabbits and the protective effects of Safltor were observed under electric telescope. Results： The intestine ultrastructure was badly injured in group I/R. Mitochondfia and intestinal mucosal cells were swellen and endoplasmic reticuhan expanded, however, in the SI group the ultrastructural injury of the ischemia greatly ameliorated. Conclusion： The ultrastructure injury occurred after intestine I/RI and Safflor has protective effects on the intestine tdtrastructure.