吸入一氧化碳防治肢体缺血／再灌注所致肝脏损伤的实验研究

目的：观察吸入外源性一氧化碳（CO）对肢体缺血／再灌注（I／R）所致肝脏损伤的防治作用。方法：健康SD大鼠100只,随机分为假手术（S）、假手术吸入CO（SC）、I／R、I／R吸入CO（RC）组。通过夹闭股动脉4h、再开放6—72h、10d复制肢体L／R致肝脏损伤模型。S、I／R组吸入普通医用空气,SC、RC组吸入含CO（体积分数为0．05％）的医用空气。光镜观察肝组织病理学变化,全自动生化分析仪检测血谷丙转氨酶（GPT）,流式细胞仪检测肝细胞凋亡百分比及bax、bcl-2的表达水平。结果：S组与SC组比较,各项观察指标无显著差别;与SC组比较,I/R及RC组肝组织呈病理改变,血清GPT及肝细胞凋亡百分比明显升高;I／R组肝细胞bax蛋白的表达水平明显升高。和L／R组相比。RC组肝组织损伤程度减轻,血清GPT、肝细胞凋亡百分比及bax蛋白的表达水平明显降低,而肝细胞bcl-2蛋白的表达水平显著升高。结论：吸入适量外源性CO对肢体I／R所致肝脏损伤有防治效应。

Exogenous carbon monoxide protects against liver injury induced by ischemia/reperfusion of hind limbs in rats

Aim： To investigate the protective effect of exogenous carbon monoxide（CO） on the liver injury induced by ischemia/reperfusion （I/R） of hind limbs in rats. Methods： 100 SD rats were divided randomly into sham operated group（S）, S＋ CO group（SC）, I/R group（I/R）, I/ R ＋ CO group （RC）. A rat model of ischemia in hind limbs and the reperfusion liver injury was established with the occlusion of the femoral arteries for 4 h and re-opening for 6 - 72 h, 10 d. The rats in SC and RC groups were exposed to air containing CO （the volume traction of CO： 0.05% ） for 2 h before and after reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The pathologic changes of liver tissue were morphologically observed by HE stain. Serum GPT activity was tested by Automatic Biochemical Analyzer. The percentage of apoptosis, expression levels of bax and bol-2 protein in liver tissue were detected by Flow Cytometry. Results： There was no difference between S and SC groups. Compared with SC group： ①Pathological changes in liver tissue were significant in I/R and RC groups. ②The serum GFF activity of I/R and RC groups was obviously increased. ③In I/R and RC groups, the percentage of apoptosis in liver tissue was all significantly increased. ④The bax expression level was significantly increased. Compared RC group with I/R group： ①Pathological change was slight. ②The serum GFF activity was depressed. ③The percentage of apoptosis and expression level of bax protein in liver tissue were depressed. ④The expression level of bcl-2 protein in liver tissue was increased. Conclusion： Exogenous CO could attenuate liver tissue injury induced by limbs I/R in rats.