Anaerobic biosynthesis of unsaturated fatty acids in the cyanobacterium,Oscillatoria limnetica |
| |
Authors: | L. L. Jahnke B. Lee M. J. Sweeney H. P. Klein |
| |
Affiliation: | (1) Planetary Biology Branch NASA, Ames Research Center, 94035 Moffett Field, CA, USA;(2) Department of Biology, Santa Clara University, 95053 Santa Clara, CA, USA |
| |
Abstract: | The mechanism for synthesis of monounsaturated fatty acids under aerobic and anaerobic conditions was studied in the facultative anaerobic cyanobacterium, Oscillatoria limnetica. The hexadecenoic acid (C161) of aerobically grown O. limnetica was shown to contain both the 7 (79%) and 9 (21%) isomers, while the octadecenoic (C181) acid was entirely the 9 acid. Incorporation of [2-14C] acetate into the fatty acids under aerobic conditions resulted in synthesis of the 7 and 9 C161 and the 9 C181. Synthesis of unsaturated fatty acids in the presence of DCMU required sulfide. Anaerobic incubations in the presence of DCMU and sulfide (less than 0.003% atmospheric oxygen) resulted in a two-fold increase in monounsaturated fatty acids of both 7 and 9 C161 and 9 and 11 C181. The synthesis of these isomers is characteristic of a bacterialtype, anaerobic pathway.Abbreviations DCMU 3(3,4-dichlorophenyl)-1,1-dimethylurea - MFA monounsaturated fatty acid |
| |
Keywords: | Cyanobacterium Oscillatoria limnetica Anacrobic unsaturated fatty acid synthesis Positional isomers |
本文献已被 SpringerLink 等数据库收录! |
|