Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes |
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Authors: | C R Shaw J N Baptist D A Wright T S Matney |
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Institution: | 1. Department of Biology, The University of Texas at Houston, M.D. Anderson Hospital and Tumor Institute, U.S.A.;2. The University of Texas at Houston Graduate School of Biomedical Sciences, Texas Medical Center, Houston, Tex. 77025 U.S.A. |
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Abstract: | A screening method was developed for detection of bacterial mutants having active enzymes with altered electrophoretic mobility. The method is based on the use of a mixture of several clones, and examination of an extract of the mixture electrophoretically. A variant enzyme will thus be detectable by its position apart from the mixture of wild-type enzymes.Following exposure to a mutagenic agent, five mutants of E. coli K12 were detected and isolated. Two of these have variant MDH (malate dehydrogenates), the others have variant forms of 6-phosphogluconate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and esterase.Preliminary mapping of the MDH locus has been performed. |
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Keywords: | MDH malate dehydrogenase MNNF PA Difco Antibiotic Medium No 3 |
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