Biological treatment of oil-contaminated sand: comparison of oil degradation based on thin-layer chromatography/flame ionization detector and respirometric analysis |
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| Authors: | Y.-S. Oh W.-Y. Choi Y.-H. Lee S.-C. Choi S.-J. Kim |
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| Affiliation: | (1) Microbiology Laboratory, Marine Biology Division, Korea Ocean Research & Development Institute, P.O. Box 29, Ansan, 425-600, Korea;(2) Division of Life Sciences, Hallym University, Chunchon, 200-702, Korea |
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| Abstract: | Oil biodegradation in oil-contaminated sand was simultaneously measured by respirometric and thin-layer chromatography/flame ionization detector (TLC/FID) methods. Degradation rate of 10–32 mg-C kg sand–1 day was achieved by amending the sand with inorganic nutrients and an oil-degrading yeast. The amendment also increased the initial CO2 production rate by 5–15 folds, which was not detected by the TLC/FID analysis. However, it was possible to monitor the accumulation of resin/asphaltene fraction up to 130% by the TLC/FID analysis during the oil degradation. |
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| Keywords: | bioremediation degradation hydrocarbon oil respirometry |
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