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| 嗜热乙醇杆菌中醛/醇脱氢酶的双启动子分析 | |
| 引用本文： | 彭惠,毛忠贵,武国干,邵蔚蓝.嗜热乙醇杆菌中醛/醇脱氢酶的双启动子分析[J].微生物学通报,2007,34(4):0727-0730. |
| 作者姓名： | 彭惠 毛忠贵 武国干 邵蔚蓝 |
| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,无锡,214036 2. 南京师范大学微生物工程重点实验室,南京,210097 3. 江南大学工业生物技术教育部重点实验室,无锡,214036;南京师范大学微生物工程重点实验室,南京,210097 |
| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
| 摘 要： | 克隆了嗜热乙醇杆菌(Thermoanaerobacter ethanolicus)中乙醇代谢的关键酶之一醛/醇脱氢酶(alcohol/acetaldehydedehy drogenase,AdhE)基因的上游假定启动子序列,并进行了结构分析。结果表明,adhE的上游序列是启动子,能启动报告基因在大肠杆菌中持续表达。首次发现adhE的启动子序列中存在两个独立的启动子(P172和P37)和核糖体结合位点(SD172和SD37),分别都具有完整功能,但其活性均低于完整的启动子序列。由此推测嗜热乙醇杆菌中adhE的表达受这两个启动子协同调控。 |
| 关 键 词： | 启动子 醛/醇脱氢酶 报告基因系统 嗜热乙醇杆菌 |
| 文章编号： | 0253-2654（2007）04-0727-04 |
| 修稿时间： | 2006-12-06 |
The Promoter Analysis of the adhE Gene Encoding the Aldehyde/alcohol Dehydrogenase in Thermoanaerobacter ethanolicus |
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| PENG Hui,MAO Zhong-Gui,WU Guo-Gan and SHAO Wei-Lan.The Promoter Analysis of the adhE Gene Encoding the Aldehyde/alcohol Dehydrogenase in Thermoanaerobacter ethanolicus[J].Microbiology,2007,34(4):0727-0730. | |
| Authors: | PENG Hui MAO Zhong-Gui WU Guo-Gan and SHAO Wei-Lan |
| Institution: | The key Laboratory of Industrial Biotechnology under Ministry of Education, Southern Yangtze University, Wuxi 214036;The key Laboratory of Industrial Biotechnology under Ministry of Education, Southern Yangtze University, Wuxi 214036;The Key Laboratory of Microbiol Engineering, Nanjing Normal University, Nanjing 210097;(1.The key Laboratory of Industrial Biotechnology under Ministry of Education, Southern Yangtze University, Wuxi 214036) (2.The Key Laboratory of Microbiol Engineering, Nanjing Normal University, Nanjing 210097) |
| Abstract: | The natural promoter of aldehyde/alcohol dehydrogenase (AdhE) gene (adhE) from Thermoanaerobacter ethanolicus was analyzed in Escherichia coli using a gene expression reporter system. The sequence immediately upstream of adhE is recognized as a constitutive promoter in E. coli, as well as being presumably an active promoter in T. ethanolicus. The natural promoter of adhE is composed of two independent promoter regions, although activities of the two independent promoter regions are much lower than that of the natural promoter. The results indicate that the AdhE synthesis is cooperatively regulated by the two promoters in T. ethanolicus. |
| Keywords: | Promoter Aldehyde/alcohol dehydrogenase Gene expression reporter system Thermoanaerobacter ethanolicus |
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