Ploidy levels of plants regenerated from mixed ploidy maize callus cultures |
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Authors: | Y. Wan L. M. McMurphy A. L. Rayburn J. M. Widholm |
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Affiliation: | (1) Department of Agronomy, Plant and Animal Biotechnology Laboratory, University of Illinois, 1201 W. Gregory, 61801 Urbana, Illinois |
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Abstract: | Summary Haploid and diploid anther-derivedZea mays callus lines were treated with the antimicrotubule herbicide pronamide to produce mixed ploidy callus as determined by flow cytometry. The ploidy levels of the plants regenerated from the callus were determined by counting the leaf epidermal guard cell chloroplast numbers. The proportion of diploid regenerated plants was somewhat lower than the proportion of diploid cells of the callus. The diploid plants regenerated somewhat faster than the haploids. The proportion of tetraploids regenerated from the pronamide treated diploid callus, which originated by spontaneous chromosome doubling, was much lower than the proportion of cells indicating that tetraploid cells survive or regenerate plants at a lower frequency than diploid cells. |
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Keywords: | anther culture callus culture chromosome doubling plant regeneration pronamide Zea mays |
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