Developmental and cytogenetic analyses of pollen sterility in Valeriana scandens L. |
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Authors: | Erica Duarte-Silva André L L Vanzela Jorge E A Mariath |
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Institution: | 1. LAVeg, Departamento de Botanica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, 91501-970, Brazil 2. LaBRE, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990, Brazil
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Abstract: | Valeriana scandens presents perfect and pistillate flowers, the latter with sterile anthers. The species is composed of two varieties with different
ploidy; V. scandens var. scandens (2n = 28) and V. scandens var. candolleana (2n = 56), both of which occur in RS, Brazil. Crosses between these varieties may give rise to hybrids with pollen sterility.
In this study, we analyzed the microsporogenesis and microgametogenesis of sterile and fertile anthers, and also investigate
whether pollen sterility is caused by an irregular meiotic process. Developmental analysis using light microscopy and scanning
electron microscopy showed that sterile anthers develop similarly to fertile anthers until the end of meiosis. After this
stage, sterile tetrads do not separate as a consequence of exine fusion between adjacent microspores, which is similar to
sterile pollen of Brassica ms-cdl1 mutants. In addition, vacuolated immature pollen grains degenerate after separation. The cytogenetic analysis of the microspore
mother cell (MMC) showed that the diploid population of V. scandens var. scandens (2n = 28) has pollen sterility that is not caused by a cytogenetic disturbance. The MMCs analyzed from prophase I to tetrad
stage showed a regular meiotic process, indicating the phenotype of V. scandens sterile pollen is a postmeiotic process formed by fusion of exine between opposite microspores. |
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