Effects of promoter,intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts |
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Authors: | Carl Rathus Robert Bower Robert G. Birch |
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Affiliation: | (1) Bureau of Sugar Experiment Stations, Brisbane, Australia;(2) Present address: CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia;(3) Department of Botany, University of Queensland, 4072, Australia |
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Abstract: | Various chimaeric promoter regions coupled to the uidA -glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts. Multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial Emu promoter relative to the CaMV 35S promoter. The relative expression strengths of promoters varied substantially between the species. Sugar-cane also differed in some respects from previously tested species in the family Poaceae. For example, in sugar-cane the nopaline synthase and CaMV 35S promoters were of equivalent strength, and insertion of Adh1 intron 1 into the 5 transcribed region decreased expression strength. |
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Keywords: | Daucus electroporation gene regulation monocot Saccharum |
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