Fluorocitrate,an Inhibitor of Glial Metabolism,Inhibits the Up-Regulation of NOS Expression,Activity and NO Production in the Spinal Cord Induced by Formalin Test in Rats |
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Authors: | Xiao-Cai Sun Wei-Na Chen Shu-Qin Li Jin-Song Cai Wen-Bin Li Xiao-Hui Xian Yu-Yan Hu Min Zhang Qing-Jun Li |
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Institution: | (1) Department of Pathophysiology, Institute of Basic Medicine, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People’s Republic of China;(2) Aging and Cognition Neuroscience Laboratory of Hebei Province, 89 Donggang Road, Shijiazhuang, 050031, People’s Republic of China |
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Abstract: | Previous experiments have suggested that nitric oxide plays an important role in nociceptive transmission in the spinal cord.
In order to explore the involvement of glia in the NO-mediated nociceptive transmission, the present study was undertaken
to investigate the effect of fluorocitrate (FC), an inhibitor of glial metabolism, on NOS expression and activity and NO production
in the spinal cord during the process of peripheral inflammatory pain and hyperalgesia induced by formalin test in rats. Sixty
adult male Sprague–Dawley rats were randomly assigned into sham, formalin, formalin + normal saline (NS), and formalin + FC
groups. The NOS expression, NOS activity and NO production was detected by NADPH-d histochemistry staining, NOS and NO assay
kit, respectively. It was found that formalin test significantly up-regulated NOS expression and activity and NO production
in the laminae I–II of the dorsal horn and the grey matter around the central canal in the lumbar spinal cord at 1 h after
the formalin test. Selective inhibition of glia metabolism with intrathecal administration of FC (1 nmol) significantly inhibited
the up-regulation in NOS expression and activity and NO production normally induced by the formalin test, which was represented
with decreases in the number and density of the NADPH-d positive cells in the dorsal horn and grey matter around the central
canal, and decrease in density of NADPH-d positive neuropil in the dorsal horn in formalin + FC group compared with formalin
group. The results suggested that glia may be involved in the NO-mediated nociceptive transmission in the spinal cord.
X.-C. Sun, W.-N. Chen and S.-Q. Li contributed equally to this work. |
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Keywords: | Nitric oxide synthase Fluorocitrate Formalin test NADPH-d staining Spinal cord |
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