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A new restriction endonuclease from Acetobacter pasteurianus. |
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| Authors: | J Seurinck A Van de Voorde M Van Montagu |
| Abstract: | A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formula; see text) and cuts at the sites indicated by the arrows. |
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