Chloride transport in human fibroblasts is activated by hypotonic shock |
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Authors: | M Rugolo T Mastocola A Flamigni G Lenaz |
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Affiliation: | Dipartimento di Biologia Evoluzionistica e Sperimentale, Universita' di Bologna, Italy. |
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Abstract: | Incubation of human skin fibroblasts in hypotonic media induced the activation of 36Cl- efflux which was roughly proportional to the decrease in the osmolality of the media. The efflux of 36Cl- was insensitive to DIDS plus furosemide and inhibited by addition of a Cl- channel blocker such as 5-nitro-2-(3-phenyl propylamino) benzoic acid (NPPB). We propose that a conductive pathway for Cl- transport, almost silent in isotonic conditions, is activated by exposing human fibroblasts to hypotonic shock, this conclusion being supported by evidence that also 36Cl- influx was enhanced by hypotonic medium. |
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