| 人骨髓间充质干细胞在精原细胞培养条件下生物学特征的观察 |
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| 引用本文： | 胡红梅,薛清彩,李伟,毛慕华,王政华,吴绍华. 人骨髓间充质干细胞在精原细胞培养条件下生物学特征的观察[J]. 中国组织化学与细胞化学杂志, 2011, 20(1): 70-74. DOI: 10.3870/zgzzhx.2011.01.016 |
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| 作者姓名： | 胡红梅 薛清彩 李伟 毛慕华 王政华 吴绍华 |
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| 作者单位： | 1. 井冈山大学医学院组织胚胎学教研室,江西吉安,343000
2. 泸州医学院组织胚胎学教研室,四川,646000 |
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| 摘 要： | 目的体外分离培养流产4h、八月龄男胎的骨髓间充质干细胞(BMSCs),并观察BMSC在精原细胞培养条件下的生物学特征,探讨人骨髓间充质干细胞诱导分化为精原细胞的可行性,为BMSCs作为"种子细胞"开辟新的途径。方法分离培养、鉴定人BMSCs,研究其在精原细胞培养条件下诱导培养BMSCs并观察其生物学特征。结果 BMSCs具有间充质细胞的特征;实验组细胞形态发生变化,免疫组化显示Oct-4和C-kit染色阳性;对照组细胞则无。结论人胎儿BMSCs有较强的增殖能力;人胎儿BMSCs在精原细胞培养条件下诱导培养,能表现出精原细胞的一些生物学特征。
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| 关 键 词： | 骨髓间充质干细胞 诱导培养 精原细胞 生物学特征 |
Biological characteristics of human bone marrow mesenchymal stem cells cultured in conditions for spermatogonia in vitro |
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| Hu Hongmei,Xue Qingcai,Li Wei,Mao Muhua,Wang Zhenghua,Wu ShaoHua. Biological characteristics of human bone marrow mesenchymal stem cells cultured in conditions for spermatogonia in vitro[J]. Chinese Journal of Histochemistry and Cytochemistry, 2011, 20(1): 70-74. DOI: 10.3870/zgzzhx.2011.01.016 |
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| Authors: | Hu Hongmei Xue Qingcai Li Wei Mao Muhua Wang Zhenghua Wu ShaoHua |
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| Affiliation: | Hu Hongmei,Xue Qingcai1,Li Wei,Mao Muhua,Wang Zhenghua,Wu ShaoHua1(Department of Histology and Embryology,School of Medicine,Jing Gang Shan University,Ji An 343000,1Department of Histology and Embryology,Luzhou Medical College,Sichuan 646000,China) |
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| Abstract: | Objective To isolate,purify and culture bone marrow mesenchymal stem cells(BMSCs) of aborted,8-month-old human male foetus and observe the main biological characteristics of BMSCs cultured in conditions for spermatogonia in vitro,so as to explore the possibility of differentiating BMSCs into spermatogonia as a new way to use BMSCs as seed cells.Methods The BMSCs were purified,cultured in conditions for spermatogonia in vitro,and identified.Results Derived BMSCs exhibited the characteristics of mesenchymal ... |
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| Keywords: | Bone marrow mesenchymal stem cells Induced cell culture Spermatogonium Biological characteristic |
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