Regulation of glucose utilization and lipogenesis in adipose tissue of diabetic and fat fed animals: Effects of insulin and manganese |
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Authors: | Najma Z Baquer M Sinclair S Kunjara Umesh C S Yadav P McLean |
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Institution: | (1) Molecular Medicine Unit, Department of Molecular Pathology, UCMS, Windeyer Building, Cleveland Street, W1P6DB London, UK;(2) Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110 067 New Delhi, India |
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Abstract: | In order to evaluate the modulatory effects of manganese, high fat diet fed and alloxan diabetic rats were taken and the changes
in the glucose oxidation, glycerol release and effects of manganese on these parameters were measured from adipose tissue.
An insulin-mimetic effect of manganese was observed in the adipose tissue in the controls and an additive effect of insulin
and manganese on glucose oxidation was seen when Mn2+ was addedin vitro. The flux of glucose through the pentose phosphate pathway and glycolysis was significantly decreased in high fat fed animals.
Although thein vitro addition of Mn2+ was additive with insulin when14CO2 was measured from control animals, it was found neither in young diabetic animals (6–8 weeks old) nor in the old (16 weeks
old). Both insulin and manganese caused an increased oxidation of carbon-1 of glucose and an increase of its incorporation
into14C-lipids in the young control animals; the additive effect of insulin and manganese suggests separate site of action. This
effect was decreased in fat fed animals, diabetic animals and old animals. Manganese alone was found to decrease glycerol
in both the control and diabetic adipose tissue inin vitro incubations. The results of the effects of glucose oxidation, lipogenesis, and glycerol release in adipose tissue of control
and diabetic animals of different ages are presented together with the effect of manganese on adipose tissue from high fat
milk diet fed animals. |
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Keywords: | Diabetes glucose utilization insulin mimetic insulin resistance manganese |
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