Hydrogen Ion Equilibria of Cajanus cajan Lectin |
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Authors: | A Salahuddin and R H Khan |
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Institution: | (1) Department of Biochemistry, J.N.M.C., and Institute of Biotechnology, A.M.U., Aligarth (U.P.), 202 002, (, India);(2) Interdisciplinary Biotechnology Unit, Aligarth Muslim University, Aligarth-, 202 002, India |
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Abstract: | Hydrogen ion titration of an affinity-purified mannose/glucose-specific lectin from Cajanus cajan pulse was carried out at 30°C and ionic strength of 0.15 by a discontinuous method. The titration was reversible in the pH range 2–12.0. The numbers of different ionizable groups per 39,000 g of the lectin were 43 carboxyl groups (pKint = 3.93), 10 imidazole groups, 21 -amino groups, 12.8 phenoxyl groups (pKint = 10.0), and 5 guanidyl groups. Only seven tyrosine residues of the lectin were dissociated under native conditions. The remaining six tyrosines became available for titration upon denaturation of the lectin in 9 M urea. |
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Keywords: | Acid– base titration legume lectin Cajanus cajan lectin hydrogen ion titration curve |
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