Extracellular matrix vesicles in rat bone after parathyroidectomy |
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| Authors: | Prof. J. Sela I. Bab A. Muhlrad U. A. Liberman D. Deutsch |
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| Affiliation: | (1) Division of Oral Pathology, The Hebrew University-Hadassah School of Dental Medicine, founded by The Alpha Omega Fraternity, Jerusalem;(2) Department of Oral Biology, The Hebrew University-Hadassah School of Dental Medicine, founded by The Alpha Omega Fraternity, Jerusalem;(3) Metabolic Unit, Beilinson Medical Center, Petach Tikva, Israel;(4) Division of Oral Pathology, Hebrew University-Hadassah, School of Dental Medicine, P.O. Box 1172, 91010 Jerusalem, Israel |
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| Abstract: | Summary The enzymatic activity of bone matrix vesicles from parathyroidectomized rats was determined and compared to the activity of vesicles from sham operated and normal animals. The vesicles were isolated from the alveolar bone by collagenase digestion and differential centrifugation and further purified on a discontinuous sucrose density gradient. The amount of extractable protein and the activity of alkaline phosphatase, acid phosphatase, and ATPase in the vesicle fractions thus obtained did not differ significantly from the values characteristic of preparations from control rats. It may therefore be suggested that parathyroid hormone depletion and the associated hypocalcemia have no significant effect on the occurrence and phosphatase activity of bone matrix vesicles. |
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| Keywords: | Bone matrix vesicles Parathyroid hormone Mineralization Rat |
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