In vitro neuroprotective action of recombinant rat erythropoietin produced by astrocyte cell lines and comparative studies with erythropoietin produced by Chinese hamster ovary cells |
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Authors: | Seiji Masuda Emi Kada Masaya Nagao Ryuzo Sasaki |
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Abstract: | In the central nervous system, astrocytes produce erythropoietin (Epo) and neurons express its receptor. To examine whether
or not the brain Epo protects the in vitro cultured neurons from glutamate-induced cell death, we established rat astrocyte
cell lines containing the plasmid for production of recombinant rat Epo. Epo partially purified from the culture medium showed
a neuroprotective effect similar to that of rat Epo produced by Chinese hamster ovary (CHO) cells. Comparison was made in
some other properties between Epo produced by these astrocyte cell lines and that by CHO cells.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | astrocyte Chinese hamster ovary cells neuroprotective action recombinant rat erythropoietin |
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