茶梅体细胞胚胎发生和植株的再生

在山茶属植物中,我们曾报道过金花茶和云南山茶组织培养形成胚状体和再生植株。茶梅(Camellia sasanqua)也是山茶属的一种重要的观赏植物,其植株矮小,花色鲜艳且具香味,除供观花外,还可做成不同造型的盆景供观赏,在园艺上也可用作茶花嫁接的砧木。茶梅组织培养通过体细胞胚状体产生植株,至今尚未见报道。现把部份实验结果报道如下。

SOMATIC EMBRYOGENESIS AND PLANTLETS REGENERATION OF CAMELLIA SASANQUA

The somatic embryoids were initiated from cotyledon segments of two cultivars of Camellia sasanqua in vitro culture on the MS medium supplemented with 6 -BA and NAA. It was obvious that the embryoids were formed directly from the surface of the cotyledons. When transferred onto a medium with 2 ppm BA, 0.5 ppm IAA, 0.2 ppm ABA and 500 ppm glutamine (GIn), the embryoids developed into complete plantlets. The growth regulator test demonstrated that GA3 had an inhibitory effect on formation of embryoids, but GA3 could stimulate shoot growth and root development of the mature embryoids. In subculture, a large number of secondary embryoids regenerated in 5 -6 weeks. The plantlets were transferred to soil where they grown normally.