Genetic variability affects the response of skeletal muscle to disuse |
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| Authors: | Camilla Reina Maroni Michael A. Friedman Yue Zhang Michael J. McClure Stefania Fulle Charles R. Farber Henry J. Donahue |
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| Affiliation: | 1.Department of Neuroscience, Imaging, and Clinical Sciences, University “G. d’Annunzio” Chieti- Pescara, Chieti, Italy;2.Institute for Engineering and Medicine and Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA;3.Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA |
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| Abstract: | Objective:To examine whether genetic variability plays a role in skeletal muscle response to disuse.Methods:We examined skeletal muscle response to disuse in five different strains of mice: CAST/EiJ, NOD/ShiLtJ, NZO/HILtJ, 129S1/SvImJ and A/J. Mice had one limb immobilized by a cast for three weeks.Results:Response to immobilization was dependent on the strain of mice. Skeletal muscle mass/body weight was decreased by immobilization in all strains except 1291/SvImJ. Immobilization decreased absolute skeletal muscle mass in quadriceps and gastrocnemius in NOD/ShiltJ and NZO/HILtJ mice. Three weeks of immobilization resulted in an increase in quadriceps levels of atrogenes in CAST/EiJ. Immobilization resulted in an increase in quadriceps and gastrocnemius levels of Myh4 in CAST/EiJ. A similar trend was observed for Myh7 in gastrocnemius muscle. Immobilization resulted in a decrease of the p-p70S6K1/total p706SK1 ratio in quadriceps of NOD/ShiLtJ mice and the gastrocnemius of A/J mice. Immobilization did not affect the p-4EBP1/total 4EBP1 ratio in quadriceps of any of the strains examined. However, the p-4EBP1/total 4EBP1 ratio in gastrocnemius was greater in immobilized, relative to control, limbs in CAST/EiJ mice.Conclusion:Genetic variability affects the response of skeletal muscle to disuse. |
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| Keywords: | Immobilization Skeletal Muscle Atrophy |
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