Sustained Formation of Focal Adhesions with Paxillin in Morphological Differentiation of PC12 Cells |
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Authors: | Sangmyung Rhee Kun Ho Lee Daegun Kim Yunhee Kim Kwon Man-Sik Kang Hyockman Kwon |
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Affiliation: | (1) Department of Molecular Biology, Dankook University, Seoul, 140-714, Korea;(2) Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul, 151-742, Korea;(3) Department of Biology, Kyung Hee University, Seoul, 130-701, Korea;(4) Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University, Seoul, 130-701, Korea |
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Abstract: | Differentiation of PC12 cells triggered by nerve growth factor (NGF) is characterized by several well-defined events including induction of a set of neuron-specific genes, gain of membrane excitability, and morphological changes such as neurite outgrowth. Here we report that K252a, a protein kinase inhibitor, converts the proliferation signal of epidermal growth factor (EGF) into the morphological differentiation signal without inducing the sustained activation of ERK and the expression of neurofilament. Major effects of EGF/K252a, found also in the NGF-treated cells, are the sustained mobility shift of paxillin in SDS-PAGE and the promoted association of Crk-II with paxillin. These effects explain the prominent and robust development of peripheral focal adhesion assembly and stress fiber-like structures observed in the early stages of PC12 cell differentiation. These results suggest a model that cytoskeletal reorganization via focal adhesion assembly triggered by NGF provides a signal required for the morphological differentiation of PC12 cells. |
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Keywords: | Differentiation EGF Focal Adhesions K252a Paxillin PC12 Cells |
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