Effect of long-term nickel ingestion on insulin binding and antilipolytic response in rat adipocytes |
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Authors: | Pilar Mayor Rafael Cabrera Bartolomé Ribas Consuelo Calle |
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Affiliation: | Department of Biochemistry, Faculty of Medicine, Madrid, Spain. |
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Abstract: | Male Wistar rats of the third generation of rats drinking 200 micrograms Ni2+/mL as NiCl2 in their drinking water were studied. Basal plasma glucose and insulin levels were unchanged. Epididymal adipocytes from Ni2(+)-fed rats showed an increased insulin binding with a slight increase in apparent insulin affinity (ED50: Ni2(+)-fed rats 2.8 x 10(-9) M and controls 5 x 10(-9) M) with no change in insulin receptor numbers (Ni2(+)-fed rats 143,000 +/- 12,000 (6) receptors/cell and controls 126,000 +/- 13,000 (5]. Moreover, a decreased sensitivity to the antilipolytic response of insulin was also observed in adipocytes from Ni2(+)-fed rats. These events could represent actions of Ni2+ both at the receptor and post-receptor insulin levels. Several possible mechanisms involved in the process are suggested. |
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Keywords: | KeywordHeading" >Index Entries Nickel rat adipocytes insulin-binding antilipolytic response |
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