Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen |
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Authors: | Laurian S Robert Sharon Allard Jean L Gerster Leslie Cass John Simmonds |
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Institution: | (1) Plant Research Centre, Central Experimental Farm, K1A 0C6 Ottawa, Ontario, Canada;(2) Present address: Centre for Food and Animal Research, Central Experimental Farm, K1A 0C6 Ottawa, Ontario, Canada |
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Abstract: | A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars. |
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Keywords: | Brassica napus pollen polygalacturonase |
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