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Isolation of glycosylated hemoglobins by a combination of ion-exchange and gel-filtration chromatographies: a pilot study
Authors:C H Lowrey  J S Soeldner
Institution:1. Chang''an University, School of Economics and Management, Nan Erhuan Road, PO Box 710064, Xi''an, People''s Republic of China;2. Xi''an Jiaotong University, School of Management, Xianning West Road, PO Box 710049, Xi''an, People''s Republic of China;3. Hong Kong Polytechnic University, Department of Management and Marketing, Faculty of Business, Hum Hong, Kowloon, Hong Kong;4. City University of Hong Kong, Department of Marketing, Tat Chee Avenue, Kowloon, Hong Kong;5. Wuhan University, School of Economics and Management, Ba Yi Road, PO Box 430072, Wuhan, People''s Republic of China;1. College of Business, University of North Texas, PO Box 311396, Denton, TX 76203-1396, United States;2. University of Dayton, Miriam Hall Room 710, 300 College Park, Dayton, OH 45469-2271, United States;3. College of Business and Entrepreneurship, University of Texas Rio Grande Valley, Edinburg, TX 78539-2999, United States
Abstract:The techniques of ion exchange and gel filtration have been combined in a single chromatographic column which allows the simultaneous isolation of hemoglobins glycosylated at their beta-amino termini from other hemoglobin species as well as from molecules differing in size from the hemoglobins. This method is unique because it makes possible isolation of preparative quantities of glycosylated hemoglobins within approximately 15 min. The method works most efficiently with a dry weight-to-weight ratio of Biorex 70 to Sephadex G25 of 1.4 to 1.0. The technique was applied to the determination of the apparent first-order rate constant for the deglycosylation of the labile form of hemoglobin AIc.
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