The Aqueous Two-Phase System Polyethylene Glycol/Dextran as a Reaction Medium for the Microsomal Monooxygenase System |
| |
Authors: | Ulrike Peter Horst A Diehl |
| |
Institution: |
a Biophysical Department, Institute of Experimental Physics, University of Bremen, Germany |
| |
Abstract: | An aqueous two-phase system of dextran and polyethylene glycol was investigated as a reaction medium for pig liver microsomes in order to find out if the partition of the microsomes, of the substrate 7-ethoxycoumarin and of the product 7-hydroxycoumarin favoured any biotechnological perspectives. Cytochrome-P-450 and NADPH-cytochrome P-450 reductase concentrations and the monooxygenase 7-ethoxycoumarin deethylation activity were measured under a variety of the system parameters. Microsomes totally partition into the bottom phase whereas the concentration ratio of substrate to product is higher in the microsome free top phase. An unfavourable effect is the specific partial deactivation of the cytochrome P-450 by polyethylene glycol. |
| |
Keywords: | Aqueous two-phase systems polyethylene glycol dextran liver microsomal monooxygenase activity membrane disturbance by polyethylene glycol |
本文献已被 InformaWorld 等数据库收录! |