Identification of amidicin: a novel peptide with C-terminal amide structure isolated from porcine cardiac atrium |
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Authors: | Ishiyama Yuichiro Kitamura Kazuo Eto Tanenao |
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Affiliation: | First Department of Internal Medicine, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. |
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Abstract: | Using a novel method employing a V8 protease digestion coupled with ethyl acetate extraction, we have purified a peptide with C-terminal amide structure from porcine cardiac atrium. The peptide was determined to be Ala-Val-Leu-Gly-Leu-CONH2. According to the sequence, we have raised an antibody and established the radioimmunoassay. Using this radioimmunoassay, we have isolated a novel 14 amino acid peptide where C-terminus was amidated. This peptide was termed amidicin. Amidicin is widely distributed in porcine tissue and is especially abundant in pituitary gland, cardiac ventricle, and spleen. |
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Keywords: | C-terminal amide Distribution Hemoglobin Peptide purification Radioimmunoassay |
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