Elimination ofM. hyorhinis from murine neuroblastoma cell lines by in vivo passage |
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Authors: | Joseph M Lombardo Karl W Lanks |
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Institution: | (1) Department of Pathology, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, 11203 Brooklyn, New York |
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Abstract: | Summary Cell lines derived from a murine neuroblastoma, Clone N18, were cured of theirM. hyorhinis infection by in vivo passage. The major variable determining success of this method was found to be the incubation time in
vivo. Infected cells maintained in vivo for 27 d or more and then placed in culture were free of mycoplasma whereas those
maintained in vivo for 7 or 14 d were found to still be infected. This approach to eliminating mycoplasma infection may be
successful using other tumor cell lines.
This work was supported in part by Grants ES01530, GM24592, and GM26167 from the National Institutes of Health, Bethesda,
MD. |
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Keywords: | mycoplasma elimination murine neuroblastoma |
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