Effect of chromium on photosystem 2 in the unicellular green alga, <Emphasis Type="Italic">Chlorella pyrenoidosa</Emphasis> |
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Authors: | Z T Hörcsik L Kovács R Láposi I Mészáros G Lakatos G Garab |
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Institution: | (1) Department of Zoology, College of Nyíregyháza, P.O.Box 166, H-4401 Nyíregyháza, Hungary;(2) Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary;(3) Department of Botany, Debrecen University, H-4032 Debrecen, Hungary |
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Abstract: | We investigated the effect of chromium (20–40 g m−3, 8–72 h) on the photosystem 2 (PS2) activities of Chlorella pyrenoidosa cells. By using chlorophyll fluorescence transients, thermoluminescence, oxygen polarography, and Western blot analysis for
D1 protein we found that inhibition of PS2 can be accounted for by the enhanced photodestruction of the reaction centres in
the cells cultivated in the presence of Cr(VI) at 25 °C in “white light” (18 W m−2). Hence photodestruction of D1 is caused by an enhanced oxidative stress and lipid peroxidation, as indicated by the appearance
of a high-temperature thermoluminescence band. |
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Keywords: | chlorophyll fluorescence D1 protein Western blot analysis thermoluminescence |
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