Optical properties of fluorochromes promising for use in biological microchips |
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| Authors: | E Yu Kotova E Ya Kreindlin V E Barsky A D Mirzabekov |
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| Institution: | (1) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia;(2) Biochip Technology Center, 60439 Argonne, IL, USA |
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| Abstract: | Fourteen fluorochromes (free and oligonucleotide-bound) and five ligands commonly used to quantitatively assess DNA duplexes
or complexes with proteins in microchips were studied by measuring their fluorescence excitation and emission spectra. The
spectral changes are described that were caused by oligonucleotide binding, solution hybridization, or varying the temperature.
The data are discussed from the standpoint of applicability and limitations of the fluorochromes and the ligands in qualitative
and quantitative assays for DNA duplexes in microchips. |
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| Keywords: | fluorescent labels fluorescently labeled oligonucleotides spectral analysis hybridization biological microchips |
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