| 褪黑素对马桑内酯致痫大鼠海马内SP水平的影响 |
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| 引用本文： | 万娜,周厚纶,赵虎,黄东辉.褪黑素对马桑内酯致痫大鼠海马内SP水平的影响[J].中国组织化学与细胞化学杂志,2009,18(6):638-644. |
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| 作者姓名： | 万娜 周厚纶 赵虎 黄东辉 |
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| 作者单位： | 华中科技大学同济医学院解剖学系,武汉,430030 |
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| 基金项目： | 华中科技大学科学研究基金 |
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| 摘 要： | 目的探讨褪黑素（melatonin,MT）对马桑内酯致痫大鼠海马内P物质水平的影响,以探讨褪黑素的抑痫作用机制。方法随机将健康成年雄性SD大鼠40只分为A、B、C、D 4组,每组10只。A组：生理盐水组;B组：马桑内酯组;C组：褪黑素＋马桑内酯组;D组：Luzindole＋褪黑素＋马桑内酯组。观察并记录行为学变化,然后分别采用免疫组织化学方法进行SP免疫组织化学染色,实时荧光定量PCR方法检测海马内SP mRNA含量变化。结果B组和D组大鼠均有不同程度的癫痫发作,而C组大鼠癫痫发作不明显,A组几乎均无发作;免疫组化结果显示,四组大鼠海马各区均有SP免疫反应阳性神经元,B组、D组与A组、C组比海马内SP免疫阳性反应明显增强（P〈0.05）,而A组与C组比无明显差异（P〉0.05）;RT-PCR结果提示,B组、D组与A组、C组相比,大鼠海马内SP mRNA明显升高（P〈0.05）,而A组与C组比无明显差异（P〉0.05）。结论MT能通过下调海马内SP水平抑制癫痫发作。
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| 关 键 词： | 癫痫 褪黑素 马桑内酯 P物质 |
EFFECT OF MELATONIN ON SP LEVEL IN HIPPOCAMPUS OF RATS WITH SEIZURE INDUCED BY CORIARIA LACTONE |
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| Wan Na,Zhou Houlun,Zhao Hu,Huang Donghui.EFFECT OF MELATONIN ON SP LEVEL IN HIPPOCAMPUS OF RATS WITH SEIZURE INDUCED BY CORIARIA LACTONE[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(6):638-644. |
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| Authors: | Wan Na Zhou Houlun Zhao Hu Huang Donghui |
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| Institution: | Wan Na,Zhou Houlun,Zhao Hu,Huang Donghui(Department of Anatomy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Objective To investigate the mechanism underlying the effects of melatonin(MT) on seizure induced by coriaria lactone,by studying its effect on substance P levels in rat hippocampus.MethodsForty healthy adult male SD rats were randomly divided into groups A,B,C and D of 10 rats each.Rats of group A were given intracerebroventricularly(i.c.v.)normal saline;rats of group B were injected with Coriaria lactone;Rats of group C were injected with MT intraperitoneally(i.P.) and Coriaria lactone;rats of group D wer... |
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| Keywords: | Epilepsy Melatonin Coriaria lactone Substance P |
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