Influence of the physiologically widespread substances on the oxidative activity of copper(II) complexes with sinefungin, a nucleoside antibiotic |
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Authors: | Mucha Ariel Cappanelli Massimo Szczepanik Wojciech Kaczmarek Piotr Ska?a Jacek Jezowska-Bojczuk Ma?gorzata |
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Institution: | Faculty of Chemistry, University of Wroc?aw, F. Joliot-Curie 14, 50383 Wroc?aw, Poland. |
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Abstract: | The oxidation-promoting reactivity of the Cu(II)-sinefungin complex in the presence of hydrogen peroxide was studied at pH 7.4, using N,N-dimethyl-p-nitrosoaniline (NDMA), as well as plasmid DNA as target molecules. Mixture of the complex with H(2)O(2) was found to be an efficient oxidant, bleaching NDMA solution, and generating single- and double-strand breaks in DNA. The oxidative DNA damage was investigated also in the presence of varying amounts of glutathione, histidine, Gly-Gly-His peptide, H2A histone, and ascorbic acid, showing diverse influence of those substances on the cleavage extension. |
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