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| 抗丙肝病毒核心抗原单克隆抗体的研制与初步鉴定 | |
| 引用本文： | 李谋深,郭满盈,李越希,李保仝,杨毓华.抗丙肝病毒核心抗原单克隆抗体的研制与初步鉴定[J].生物技术通讯,2000,11(1):20-22. |
| 作者姓名： | 李谋深 郭满盈 李越希 李保仝 杨毓华 |
| 作者单位： | 1. 解放军第98医院检验科,湖州,313000 2. 南京军区联勤部军事医学研究所,南京,210002 3. 南京军区南京总医院全军医学检验中心,南京,210002 |
| 摘 要： | 用基因工程重组技术获得的丙肝病毒（ＨＣＶ）核心蛋白抗原与鼠血清白蛋白交联后免疫Ｂａｌｂ／ｃ小鼠，用杂交瘤技术成功地建立了４株稳定分泌抗核心抗原单克隆抗体的杂交瘤细胞，试验结果表明，该４株ＭｃＡｂｓ与免疫抗原及核心区Ｃ３３肽、ＣＰ９、ＣＰ１０抗原有较强的抗原－抗体反应，与ＨＣＶ ＮＳ３、ＮＳ４、ＮＳ５无反应，在竞争ＥＬＩＳＡ中，对ＨＣＶ－ＩｇＧ阳性血清有较好的抑制作用。４株ＭｃＡｂｓ中３株为ＩｇＧ２ |
| 关 键 词： | 丙型肝炎病毒 核心抗原 单克隆抗体 基因工程 |
Preparation of monoclonal antibodies to HCV core antigen and their characteristic study |
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| Li Muoshen,Guo Manying,Li Yuexi,Li Baotong,Yang Yuhua.Preparation of monoclonal antibodies to HCV core antigen and their characteristic study[J].Letters in Biotechnology,2000,11(1):20-22. | |
| Authors: | Li Muoshen Guo Manying Li Yuexi Li Baotong Yang Yuhua |
| Abstract: | Bal b/c mice were immunized with recombinant HCV core antigen and coupled with murine serum albumin. Four monoclonal antibodies (1F5, 2C5, 1D10, 2G2) with practical value were obtained by hybridoma technique. The reactions of the 4 McAbs with the recombinant core antigen and core antigen C33 peptide, CP9, CP10 were strong, but no reactions were detectable with HCV NS3, NS4, NS5. The 4 McAbs inhibited anti-HCV-IgG positive sera in competitive ELISA. The results also showed that 3 of the 4 monoclonal antibodies were IgG2a and one was IgM in nature. |
| Keywords: | hepatitis C virus core antigen monoclonal antibody |
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