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Some properties of deoxyribonucleic acid preparations from Chilo, Sericesthis and Tipula iridescent viruses
Authors:A J Bellett
Affiliation:1. Instituto de Informática, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, Porto Alegre, RS 9500, Brazil;2. ROGLE - Departamento Organización de Empresas, Universitat Politècnica de València, Spain;1. Department of Materials Science, Montanuniversität Leoben, Franz-Josef-Strasse 18, 8700 Leoben, Austria;2. Helmholtz-Zentrum Geesthacht, Max-Planck-Strasse 1, 21502 Geesthacht, Germany;3. voestalpine Stahl GmbH, voestalpine-Strasse 3, 4020 Linz, Austria
Abstract:The amount of DNA in the particles of three viruses of the iridescent virus group has been estimated, and some properties of DNA preparations from the viruses have been investigated. All the viruses contain double-stranded DNA. The DNA of Chilo iridescent virus contains 28 to 29% G + C base pairs, that of Sericesthis iridescent virus 31% G + C and that of Tipula iridescent virus 32% G + C. The differences in base ratios were confirmed by analysis of mixed DNA bands at equilibrium in CsCl. The contents of DNA per virus particle (expressed in molecular weight units) and the molecular weights of extracted DNA were estimated to be 135 million and 130 million for Chilo iridescent virus, 147 million and 134 million for Sericesthis iridescent virus and 155 million and 126 million for Tipula iridescent virus, respectively.
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