Rapid mapping of cosmid clones on pig chromosomes by fluorescence in situ hybridization |
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| Authors: | M. Yerle A. Goureau J. Gellin P. Le Tissier C. Moran |
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| Affiliation: | (1) Laboratoire de Génétique Cellulaire, INRA, BP 27, 31326 Castanet-Tolosan, France;(2) Department of Animal Science, The University of Sydney, 2006, New South Wales, Australia;(3) Ecole Nationale de Formation Agronomique, BP 87, 31326 Castanet-Tolosan, France |
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| Abstract: | Nineteen cosmids have been mapped to pig chromosomes by fluorescence in situ hybridization. Two kinds of cosmid clones were isolated as potential physical and genetic markers for the pig genome. Anonymous cosmids were obtained by screening a commercial cosmid library and were localized to Chromosomes (Chrs) 1, 2, 6, 7, 8, 10, 11, 12, 13, and 14. Some of these cosmids were found to reveal RFLP type DNA polymorphism. Microsatellite-containing cosmid clones were isolated by screening a pig cosmid library with a (CA)10 probe and were regionally mapped to Chrs 2, 6, 7, 13, and 14. Ten of the 19 chromosomes in the pig were labeled with these probes. Two-color fluorescence in situ hybridization was used to increase the efficiency of the cosmid localizations. |
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