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Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast |
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| Authors: | Misa?Gray,Martin?Kupiec,Saul?M?Honigberg author-information" > author-information__contact u-icon-before" > mailto:hongibergs@umkc.edu" title=" hongibergs@umkc.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
| Affiliation: | (1) Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA;(2) Dept. of Molecular Micro & Biotech, Tel Aviv University, Ramat Aviv, 69978, Israel |
| Abstract: | BackgroundA valuable weapon in the arsenal available to yeast geneticists is the ability to introduce specific mutations into yeast genome. In particular, methods have been developed to introduce deletions into the yeast genome using PCR fragments. These methods are highly efficient because they do not require cloning in plasmids. |
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