Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein |
| |
Authors: | Huang Qiulong Yu Liping Petros Andrew M Gunasekera Angelo Liu Zhihong Xu Nan Hajduk Philip Mack Jamey Fesik Stephen W Olejniczak Edward T |
| |
Institution: | Global Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Illinois 60064-6098, USA. |
| |
Abstract: | The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|