Bradykinin stimulates the production of prostaglandin E2 and interleukin-6 in human osteoblast-like cells. |
| |
| Authors: | S Rahman R A Bunning P R Dobson D B Evans K Chapman T H Jones B L Brown R G Russell |
| |
| Institution: | Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, UK. |
| |
| Abstract: | The effect of bradykinin (BK) on proteinase activity, prostaglandin synthesis, and the production of interleukin-6 (IL-6) was investigated in cultures of human osteoblast-like cells. Bradykinin had no effect on stromelysin activity and plasminogen activator activity produced by human osteoblast-like cells. However, BK stimulated the production of prostaglandin E2, an effect that was markedly enhanced by pre-incubation with recombinant interleukin-1 alpha (rhIL-1 alpha), but was apparently unaffected by BK receptor antagonists types 1 and 2. Bradykinin stimulated the intracellular accumulation of total inositol phosphates suggesting that its effects were mediated by stimulation of phosphoinositide metabolism. Bradykinin within the dose range of 10(-11)-10(-5) M also significantly stimulated the production of IL-6. Bradykinin may, therefore, mediate a variety of responses in bone under both physiological and pathological conditions. |
| |
| Keywords: | |
|
|
