Glutamate-induced cobalt uptake elicited by kainate receptors in rat taste bud cells |
| |
Authors: | Chung Ki-Myung Lee Sang-Bok Heur Ryang Cho Young-Kyung Lee Cil-Han Jung Hye-Young Chung Se-Hwan Lee Seung-Pyo Kim Kyung-Nyun |
| |
Affiliation: | Department of Physiology and Neuroscience, College of Dentistry, Kangnung National University, 123 Chibyun-dong, Kangnung, Kangwon-do, 210-702, Republic of Korea. |
| |
Abstract: | Glutamate-induced cobalt uptake reveals non-N-methyl-D-aspartate (non-NMDA) glutamate receptors (GluRs) in rat taste bud cells. However, it is not known which type of non-NMDA glutamate receptors is involved. We used a cobalt staining technique combined with pharmacological tests for kainate or alpha-amino-3-hydroxy-5-methyl-isoxazole-propionic acid (AMPA) receptors and/or immunohistochemistry against subunits of GluRs to examine the presence of non-NMDA receptors in rat foliate tastebud cells. Cobalt uptake into taste cells was elicited by treating taste buds with glutamate, kainate or SYM 2081, a kainate receptor agonist. Treating taste buds with AMPA or fluorowillardiine did not stimulate significant cobalt uptake. Moreover, 6-cyano-7-nitro-quinoxaline-2, 3-dione significantly reduced cobalt staining elicited by glutamate or kainate receptor agonists, but SYM 2206, an AMPA receptor antagonist, did not. Immunohistochemistry against subunits of GluRs reveals GluR6 and KA1-like immunoreactivity. Moreover, most glutamate-induced cobalt-stained cells showed GluR6 and KA1-like immunoreactivity. These results suggest that glutamate-induced cobalt uptake in taste cells occurs mainly via kainate type GluRs. |
| |
Keywords: | AMPA cobalt staining foliate papilla glutamate receptors kainate taste bud |
本文献已被 PubMed Oxford 等数据库收录! |
|